Part:BBa_K1897006

Has operon promoter

Usage and Biology

The Has promoter is used for gene expression control, where expression of genes downstream to the Has promoter is triggered by the Has system. The Has system originates from the Serratia marcescens where it functions as a heme acquisition system. The core signalling molecules of the system are the hemophore HasA, receptor HasR, anti-sigma factor HasS and extra cytoplasmic function sigma factor HasI. HasA detects and binds to heme and the holo-HasA can bind to HasR on the cell surface. This induces a conformational change and activation of HasS. HasS then releases the sigma factor HasI and this will allow for the expression of genes under the control of the Has promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]